Yeast viral RNA polymerase is a transcriptase

A viral deubiquitylating enzyme stabilizes viral RNA- dependent RNA polymerase and affects viral infectivity

Thank you for submitting your manuscript on the identification of DUB function in the TYMV protease for consideration by The EMBO Journal. Three referees have now provided their comments, which are copied below. As you will see, the referees appreciate that your study (for the most part convincingly) establishes TYMV 98K as a bona fide new example of a viral DUB enzyme, although a few open ques...

A viral deubiquitylating enzyme targets viral RNA-dependent RNA polymerase and affects viral infectivity.

Selective protein degradation via the ubiquitin-proteasome system (UPS) plays an essential role in many major cellular processes, including host-pathogen interactions. We previously reported that the tightly regulated viral RNA-dependent RNA polymerase (RdRp) of the positive-strand RNA virus Turnip yellow mosaic virus (TYMV) is degraded by the UPS in infected cells, a process that affects viral...

A viral deubiquitylating enzyme targets viral RNA - dependent RNA polymerase and affects viral

The RNA polymerase of influenza a virus is stabilized by interaction with its viral RNA promoter.

The RNA polymerase of the influenza virus is responsible for the transcription and replication of the segmented RNA viral genome during infection of host cells. Polymerase function is known to be strictly dependent on interaction with its RNA promoter, but no attempts to investigate whether the virion RNA (vRNA) promoter stabilizes the polymerase have been reported previously. Here we tested wh...

Splicing-Dependent RNA Polymerase Pausing in Yeast

In eukaryotic cells, there is evidence for functional coupling between transcription and processing of pre-mRNAs. To better understand this coupling, we performed a high-resolution kinetic analysis of transcription and splicing in budding yeast. This revealed that shortly after induction of transcription, RNA polymerase accumulates transiently around the 3' end of the intron on two reporter gen...